crispri

What a genome-scale CRISPRi screen can and cannot discover. 50 Hallmark programs x 9,837 knockdowns in K562. Four evaluations, three negative, all four reported.

🌱 Designing CRISPRi experiments in bacteria

Reproducible CRISPRi/Perturb-seq analysis of an ENTPD1/CD39 T-cell program in rheumatoid arthritis and methotrexate treatment

Repository containing the code and methods for Bosch & DeJesus et al. 2021

PyTorch classifier predicting CRISPRi guide identity from single-cell expression (Adamson et al. 2016 Perturb-seq)

Computational design and adversarial validation of synthetic gene circuits targeting PDAC transcription factors, trained from scratch on real public genomics data

Adversarial-debate LLM agent for predicting CRISPRi perturbation effects on gene expression (Genentech MLGenX BioReasoning Challenge)

AI-assisted platform for designing CRISPR experiments — from target selection to bench-ready protocols

Phenotypic screening of a CRISPRi strain library [Smith et 2017, Mol Syst Biol (2017)13:913] for acetic acid tolerance targeting essential and respiratory essential genes in Saccharomyces cerevisiae

Figure code and data for Johnson et al. 2021, J Am Chem Soc 143(31):12003-12013, doi:10.1021/jacs.1c02622. CRISPRi chemical genomics identifying IleRS as the target of thiomarinol